Determination of Pneumococcal Serotypes in Meningitis Cases in Niger, 2003–2011 - Archive ouverte HAL Access content directly
Journal Articles PLoS ONE Year : 2013

Determination of Pneumococcal Serotypes in Meningitis Cases in Niger, 2003–2011

(1) , (1) , (1)
1

Abstract

Background The epidemiology of pneumococcal meningitis in the African ‘meningitis belt’ is poorly studied. In order to ensure an effective vaccination strategy and post-vaccination surveillance, we examined the serotype distribution patterns of pneumococcal meningitis in Niger over the period 2003–2011. Methods Cerebrospinal fluid (CSF) samples were collected from different health facilities throughout Niger in the frame of the national microbiological surveillance of meningitis. Determination of the serotype of CSF positive for pneumococci was performed using a sequential multiplex PCR method (SM-PCR) adapted with a national algorithm in which 32 different serotypes were covered and grouped into eight consecutive PCR. Results The SM-PCR assay could predict the Sp serotype for 779 CSF (88.7%), 98 CSF (11.3%) were not-typeable in our national-adapted algorithm. In total, 26 different serotypes were identified. Serotype 1 (n = 393) was the most prevalent and accounted for 45.3% of infections, followed by serogroups/serotypes 12F/(12A)/(44)/(46) (7.3%), 6/(6A/6B/6C/6D) (5.4%), 14 (5.2%), 5 (4.6%), 23F (4.2%), 45 (3.6%), 2 (3.1%), 18/(18A/18B/18C/18F) (2.9%) and 17 others serotypes with a prevalence of less than 2%. The proportion of serotype 1 in infants(<2 years old) represented only 4.3% of the cases affected by this serotype. In contrast, serotypes 5, 6, 14, 19A and 23F were only detected in very young children. Conclusions The proportion of serotype 1 in the pneumococcal meningitis cases and the theoretical vaccine coverage across all age groups advocates for the introduction of a conjugate vaccine (PCV10 or 13) into the Expanded Programme on Immunization (EPI) in Niger. Post-vaccine introduction surveillance supported by molecular approaches will be essential to provide a comprehensive picture of the impact of the vaccine on the burden reduction of pneumococcal meningitis and on pneumococcal serotype distribution.
Fichier principal
Vignette du fichier
pone.0060432.pdf (769.63 Ko) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

pasteur-03238558 , version 1 (27-05-2021)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Jean-Marc Collard, Abdel-Kader Alio Sanda, Jean-François Jusot. Determination of Pneumococcal Serotypes in Meningitis Cases in Niger, 2003–2011. PLoS ONE, 2013, 8 (3), pp.e60432. ⟨10.1371/journal.pone.0060432⟩. ⟨pasteur-03238558⟩

Collections

RIIP RIIP_CERMES
12 View
18 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More