A prospective cohort study of Cutaneous Leishmaniasis due to Leishmania major: Dynamics of the Leishmanin skin test and its predictive value for protection against infection and disease

Jihène Bettaieb (1, 2, 3) , Amine Toumi (1, 3) , Wissem Ghawar (1, 2, 3) , Sadok Chlif (1, 2, 3) , Mariem Nouira (1, 3) , Nabil Belhaj-Hamida (1, 2, 3) , Adel Gharbi (1, 2, 3) , Nissaf Ben-Alaya (4) , Dhafer Laouini (2, 3) , Hechmi Louzir (2, 3) , Koussay Dellagi (2, 3) , Afif Ben Salah (1, 2, 3, 5)

1 Laboratoire d'Epidémiologie Médicale, Institut Pasteur de Tunis
2 LR11IPT02 - Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection
3 UTM - Université de Tunis El Manar
4 ONMNE - Observatoire national des maladies nouvelles et émergentes [Tunis]
5 Arabian Gulf University

Background Leishmanin Skin Test (LST) is considered as a useful indicator of past infection by Leishmania parasites. However, the temporal dynamics of a positive LST under different epidemiologic scenarios and whether it relates to the protection against the recurrence of an overt disease are not fully documented. Methodology/Principal findings We report here on a population based prospective study conducted on 2686 individuals living in two foci located in Central Tunisia, to assess over a one-year epidemiologic season, the incidence of Leishmania (L.) major infection and disease and changes in LST reactivity. The two foci were both endemic for Cutaneous Leishmaniasis (CL) due to L. major, but contrasted in their history for this disease (ie: an old focus versus a recent focus). We found that most infections occurred in the new focus (290/1000; 95% CI: 265–315 person-years) with an incidence rate of CL lesions 2.4 times higher than in the old focus. Likewise, the rates of LST reactivity reversion and loss, in the new focus, were 99/1000[38–116] person-years and 14/1000[8–21] person-years, respectively. Loss of LST reactivity was not noticed in the old focus. Interestingly, the incidence rates of symptomatic infection did not differ significantly according to the LST status at enrolment (negative versus positive) between the combined foci and the new one. Conclusions/Significance Our findings confirm LST as a good tool for assessing L. major cryptic infection. However, the instability of the LST positivity in new foci should be considered as an important confounder of the outcome of this infection when developing a research protocol for vaccine trial.

Domaines: Sciences du Vivant [q-bio]

Jihène Bettaieb, Amine Toumi, Wissem Ghawar, Sadok Chlif, Mariem Nouira, et al.. A prospective cohort study of Cutaneous Leishmaniasis due to Leishmania major: Dynamics of the Leishmanin skin test and its predictive value for protection against infection and disease. PLoS Neglected Tropical Diseases, 2020, 14 (8), pp.e0008550. ⟨10.1371/journal.pntd.0008550⟩. ⟨pasteur-03542131⟩