Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet Disease

Olfa Maghrebi (1, 2) , Mariem Hanachi (3, 4) , Khadija Bahrini (1, 2) , Mariem Kchaou (2, 5) , Cyrine Jeridi (2, 6) , Samir Belal (2, 6) , Samia Ben Sassi (2, 6) , Mohamed-Ridha Barbouche (1, 2) , Oussama Souiai (2, 3) , Meriam Belghith (1, 2)

1 LR11IPT02 - Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection
2 UTM - Université de Tunis El Manar
3 LR11IPT09 - Laboratoire de Bioinformatique, biomathématiques, biostatistiques (BIMS)
4 UCAR - Université de Carthage (Tunisie)
5 Hôpital Charles Nicolle [Tunis]
6 Institut National de Neurologie Mongi-Ben Hamida - Mongi Ben Hmida National Institute of Neurology [Tunis]

Inflammatory demyelinating disorders of the central nervous system are debilitating conditions of the young adult, here we focus on multiple sclerosis (MS) and neuro-Behçet disease (NBD). MS is an autoimmune disorder of the central nervous system. NBD, a neurological manifestation of an idiopathic chronic relapsing multisystem inflammatory disease, the behçet disease. The diagnosis of MS and NBD relies on clinical symptoms, magnetic resonance imaging and laboratory tests. At first onset, clinical and imaging similarities between the two disorders may occur, making differential diagnosis challenging and delaying appropriate management. Aiming to identify additional discriminating biomarker patterns, we measured and compared gene expression of a broad panel of selected genes in blood and cerebrospinal fluid (CSF) cells of patients suffering from NBD, MS and non inflammatory neurological disorders (NIND). To reach this aim, bivariate and multivariate analysis were applied. The Principal Analysis Component (PCA) highlighted distinct profiles between NBD, MS, and controls. Transcription factors foxp3 in the blood along with IL-4, IL-10, and IL-17 expressions were the parameters that are the main contributor to the segregation between MS and NBD clustering. Moreover, parameters related to cellular activation and inflammatory cytokines within the CSF clearly differentiate between the two inflammatory diseases and the controls. We proceeded to ROC analysis in order to identify the most distinctive parameters between both inflammatory neurological disorders. The latter analysis suggested that IL-17, CD73 in the blood as well as IL-1β and IL-10 in the CSF were the most discriminating parameters between MS and NBD. We conclude that combined multi-dimensional analysis in blood and CSF suggests distinct mechanisms governing the pathophysiology of these two neuro-inflammatory disorders.

Mots clés: multiple sclerosis; neuro-Behçet disease; multivariate analysis; cerebrospinal fluid; IL-17; CD73; A2A receptor

Domaines: Sciences du Vivant [q-bio]

Olfa Maghrebi, Mariem Hanachi, Khadija Bahrini, Mariem Kchaou, Cyrine Jeridi, et al.. Differential Gene Expression Patterns in Blood and Cerebrospinal Fluid of Multiple Sclerosis and Neuro-Behçet Disease. Frontiers in Genetics, 2021, 12, pp.638236. ⟨10.3389/fgene.2021.638236⟩. ⟨pasteur-03545414⟩