Anti-Cancer Effect of Moroccan Cobra Naja haje Venom and Its Fractions against Hepatocellular Carcinoma in 3D Cell Culture - Archive ouverte HAL Access content directly
Journal Articles Toxins Year : 2021

Anti-Cancer Effect of Moroccan Cobra Naja haje Venom and Its Fractions against Hepatocellular Carcinoma in 3D Cell Culture

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Imane Gourja
  • Function : Author
  • PersonId : 1124605
Fatima Chgoury
  • Function : Author
  • PersonId : 1124607
Salma Chakir
  • Function : Author
  • PersonId : 1124609
Rachida Cadi
  • Function : Author
  • PersonId : 1124610
Khadija Mounaji
  • Function : Author
  • PersonId : 1124611

Abstract

Hepatocellular carcinoma (HCC) is the most common primary liver cancer in adults, the fifth most common malignancy worldwide and the third leading cause of cancer related death. An alternative to the surgical treatments and drugs, such as sorafenib, commonly used in medicine is necessary to overcome this public health problem. In this study, we determine the anticancer effect on HCC of Moroccan cobra Naja haje venom and its fraction obtained by gel filtration chromatography against Huh7.5 cancer cell line. Cells were grown together with WI38 human fibroblast cells, LX2 human hepatic stellate cell line, and human endothelial cells (HUVEC) in MCTS (multi-cellular tumor spheroids) models. The hepatotoxicity of venom and its fractions were also evaluated using the normal hepatocytes cell line (Fa2N-4 cells). Our results showed that an anti HCC activity of Moroccan cobra Naja haje venom and, more specifically, the F7 fraction of gel filtration chromatography exhibited the greatest anti-hepatocellular carcinoma effect by decreasing the size of MCTS. This effect is associated with a low toxicity against normal hepatocytes. These results strongly suggest that the F7 fraction of Moroccan cobra Naja haje venom obtained by gel filtration chromatography possesses the ability to inhibit cancer cells proliferation. More research is needed to identify the specific molecule(s) responsible for the anticancer effect and investigate their mechanism of action.
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Origin : Publication funded by an institution

Dates and versions

pasteur-03546817 , version 1 (28-01-2022)

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Attribution - CC BY 4.0

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Ayoub Lafnoune, Su-Yeon Lee, Jin-Yeong Heo, Imane Gourja, Bouchra Darkaoui, et al.. Anti-Cancer Effect of Moroccan Cobra Naja haje Venom and Its Fractions against Hepatocellular Carcinoma in 3D Cell Culture. Toxins, 2021, 13 (6), pp.402. ⟨10.3390/toxins13060402⟩. ⟨pasteur-03546817⟩
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