Bat coronaviruses related to SARS-CoV-2 and infectious for human cells - Institut Pasteur du Laos Accéder directement au contenu
Article Dans Une Revue Nature Année : 2022

Bat coronaviruses related to SARS-CoV-2 and infectious for human cells

Vincent Lacoste
Félix Rey

Résumé

The animal reservoir of SARS-CoV-2 is unknown despite reports of SARS-CoV-2-related viruses in Asian Rhinolophus bats1-4, including the closest virus from R. affinis, RaTG13 (refs. 5,6), and pangolins7-9. SARS-CoV-2 has a mosaic genome, to which different progenitors contribute. The spike sequence determines the binding affinity and accessibility of its receptor-binding domain to the cellular angiotensin-converting enzyme 2 (ACE2) receptor and is responsible for host range10-12. SARS-CoV-2 progenitor bat viruses genetically close to SARS-CoV-2 and able to enter human cells through a human ACE2 (hACE2) pathway have not yet been identified, although they would be key in understanding the origin of the epidemic. Here we show that such viruses circulate in cave bats living in the limestone karstic terrain in northern Laos, in the Indochinese peninsula. We found that the receptor-binding domains of these viruses differ from that of SARS-CoV-2 by only one or two residues at the interface with ACE2, bind more efficiently to the hACE2 protein than that of the SARS-CoV-2 strain isolated in Wuhan from early human cases, and mediate hACE2-dependent entry and replication in human cells, which is inhibited by antibodies that neutralize SARS-CoV-2. None of these bat viruses contains a furin cleavage site in the spike protein. Our findings therefore indicate that bat-borne SARS-CoV-2-like viruses that are potentially infectious for humans circulate in Rhinolophus spp. in the Indochinese peninsula.
Fichier principal
Vignette du fichier
Nature bats Laos.pdf (37.02 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

pasteur-03661247 , version 1 (19-05-2022)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Sarah Temmam, Khamsing Vongphayloth, Eduard Baquero, Sandie Munier, Massimiliano Bonomi, et al.. Bat coronaviruses related to SARS-CoV-2 and infectious for human cells. Nature, 2022, 604 (7905), pp.330-336. ⟨10.1038/s41586-022-04532-4⟩. ⟨pasteur-03661247⟩
1059 Consultations
155 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More