Molecular analysis of isoniazid-resistant Mycobacterium tuberculosis isolates in Uruguay.

G. Greif (1) , C. Coitinho (2) , C. Rivas (2) , J. van Ingen (3) , C. Robello (1, 4)

1 Institut Pasteur de Montevideo
2 Departamento de Laboratorio
3 Department of Clinical Microbiology
4 Departamento de Bioquímica

The incidence of tuberculosis (TB) is increasing in high-risk populations in Uruguay, possibly owing to emerging resistance. Mycobacterial interspersed repetitive units (MIRU) genotyping and katG sequence analysis of isoniazid (INH) resistance-associated mutations were performed in 45 INH-resistant Mycobacterium tuberculosis isolates in Uruguayan patients. The genotype distribution among INH-resistant isolates shares features of that of neighbouring countries, with a predominance of Latin American and Mediterranean, T and Haarlem genotypes, although the S genotype was particularly frequent among our isolates. Forty-four per cent of INH-resistant strains harboured the S315T mutation in katG; we found novel katG mutations (W321X, G269T, P232R and G221Wfs1) that could explain INH resistance.

Domaines: Sciences du Vivant [q-bio]

G. Greif, C. Coitinho, C. Rivas, J. van Ingen, C. Robello. Molecular analysis of isoniazid-resistant Mycobacterium tuberculosis isolates in Uruguay.. International Journal of Tuberculosis and Lung Disease, 2012, 16 (7), pp.947-9. ⟨10.5588/ijtld.11.0559⟩. ⟨pasteur-00839356⟩