Upregulation of TPX2 by STAT3: Identification of a Novel STAT3 Binding Site

Rossana Cocchiola (1) , Caterina Grillo (1) , Fabio Altieri (1) , Silvia Chichiarelli (1) , Carlo Turano (1) , Margherita Eufemi (1)

1 Department of Biochemical Sciences "Rossi Fanelli"

TPX2, a protein involved in mitosis, is considered a good marker for actively proliferating tissues, highly expressed in a number of cancer cells. We show the presence of high-affinity binding site for STAT3 in the 59-flanking region of the Tpx2 gene, which is in vivo bound by activated STAT3. A specific STAT3 peptide inhibitor represses the expression of the Tpx2 gene and inhibits the binding of STAT3 to its consensus sequence in human cell lines where STAT3 is activated. These results indicate that activated STAT3 contributes to the over-expression of Tpx2 through the binding to an enhancer site.

Domaines: Sciences du Vivant [q-bio]; Biochimie, Biologie Moléculaire

Rossana Cocchiola, Caterina Grillo, Fabio Altieri, Silvia Chichiarelli, Carlo Turano, et al.. Upregulation of TPX2 by STAT3: Identification of a Novel STAT3 Binding Site. PLoS ONE, 2014, pp.7. ⟨10.1371/journal.pone.0113096⟩. ⟨pasteur-01148080⟩